David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Sakai, H., et al. “Isoflurane provides sustained neuroprotection in rats subjected to focal ischemia.” Journal of Neurosurgical Anesthesiology, vol. 18, no. 4, Jan. 2006, p. 281. Scopus, doi:10.1097/00008506-200610000-00030. Full Text

Klein, Stephen M., et al. “Peripheral nerve block techniques for ambulatory surgery.Anesth Analg, vol. 101, no. 6, Dec. 2005, pp. 1663–76. Pubmed, doi:10.1213/01.ANE.0000184187.02887.24. Full Text

Wise-Faberowski, Lisa, et al. “Isoflurane-induced neuronal degeneration: an evaluation in organotypic hippocampal slice cultures.Anesth Analg, vol. 101, no. 3, Sept. 2005, pp. 651–57. Pubmed, doi:10.1213/01.ane.0000167382.79889.7c. Full Text

Lynch, John R., et al. “Simvastatin reduces vasospasm after aneurysmal subarachnoid hemorrhage: results of a pilot randomized clinical trial.Stroke, vol. 36, no. 9, Sept. 2005, pp. 2024–26. Pubmed, doi:10.1161/01.STR.0000177879.11607.10. Full Text

Abraini, Jacques H., et al. “Postischemic nitrous oxide alone versus intraischemic nitrous oxide in the presence of isoflurane: what it may change for neuroprotection against cerebral stroke in the rat.Anesth Analg, vol. 101, no. 2, Aug. 2005, p. 614. Pubmed, doi:10.1213/01.ANE.0000159020.86959.83. Full Text

Keifer, John C., et al. “A retrospective analysis of a remifentanil/propofol general anesthetic for craniotomy before awake functional brain mapping.Anesth Analg, vol. 101, no. 2, Aug. 2005, pp. 502–08. Pubmed, doi:10.1213/01.ANE.0000160533.51420.44. Full Text

McAdoo, Jessica D., et al. “Intrathecal administration of a novel apoE-derived therapeutic peptide improves outcome following perinatal hypoxic-ischemic injury.Neurosci Lett, vol. 381, no. 3, June 2005, pp. 305–08. Pubmed, doi:10.1016/j.neulet.2005.02.036. Full Text

Warner, D. S. “Anesthetics as neuroprotectants/neurotoxins.” Anesthesia and Analgesia, vol. 100, no. 3 SUPPL., Mar. 2005, pp. 95–99.

McKee, J. Andrew, et al. “Analysis of the brain bioavailability of peripherally administered magnesium sulfate: A study in humans with acute brain injury undergoing prolonged induced hypermagnesemia.Crit Care Med, vol. 33, no. 3, Mar. 2005, pp. 661–66. Pubmed, doi:10.1097/01.ccm.0000156293.35868.b2. Full Text

Lynch, John R., et al. “A novel therapeutic derived from apolipoprotein E reduces brain inflammation and improves outcome after closed head injury.Exp Neurol, vol. 192, no. 1, Mar. 2005, pp. 109–16. Pubmed, doi:10.1016/j.expneurol.2004.11.014. Full Text

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