David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

McKee, J. Andrew, et al. “Magnesium neuroprotection is limited in humans with acute brain injury.Neurocrit Care, vol. 2, no. 3, 2005, pp. 342–51. Pubmed, doi:10.1385/NCC:2:3:342. Full Text

Lascola, Christopher D., et al. “Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice.Ajr Am J Roentgenol, vol. 183, no. 6, Dec. 2004, pp. 1791–97. Pubmed, doi:10.2214/ajr.183.6.01831791. Full Text

Wise-Faberowski, Lisa, et al. “Apoptosis is not enhanced in primary mixed neuronal/glial cultures protected by isoflurane against N-methyl-D-aspartate excitotoxicity.Anesth Analg, vol. 99, no. 6, Dec. 2004, pp. 1708–14. Pubmed, doi:10.1213/01.ANE.0000136474.35627.FF. Full Text

Warner, David S., and Cecil O. Borel. “Treatment of traumatic brain injury: one size does not fit all.Anesth Analg, vol. 99, no. 4, Oct. 2004, pp. 1208–10. Pubmed, doi:10.1213/01.ANE.0000139930.04010.0D. Full Text

Warner, David S. “Anesthetics provide limited but real protection against acute brain injury.J Neurosurg Anesthesiol, vol. 16, no. 4, Oct. 2004, pp. 303–07. Pubmed, doi:10.1097/00008506-200410000-00011. Full Text

Yokoo, Noriko, et al. “Intraischemic nitrous oxide alters neither neurologic nor histologic outcome: a comparison with dizocilpine.Anesth Analg, vol. 99, no. 3, Sept. 2004, pp. 896–903. Pubmed, doi:10.1213/01.ANE.0000132973.32387.8B. Full Text

Sheng, Huaxin, et al. “Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy.Neurosci Lett, vol. 366, no. 2, Aug. 2004, pp. 220–25. Pubmed, doi:10.1016/j.neulet.2004.05.050. Full Text

Warner, David S., et al. “Oxidants, antioxidants and the ischemic brain.J Exp Biol, vol. 207, no. Pt 18, Aug. 2004, pp. 3221–31. Pubmed, doi:10.1242/jeb.01022. Full Text

Homi, H. Mayumi, et al. “Neuroprotection by Nitrous Oxide and Xenon and Its Relation to Minimum Alveolar Concentration.” Anesthesiology, vol. 101, no. 1, Ovid Technologies (Wolters Kluwer Health), July 2004, pp. 261–261. Crossref, doi:10.1097/00000542-200407000-00050. Full Text

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