David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Selected Grants

Same awarded by National Institutes of Health (Principal Investigator). 2000 to 2003

Apolipoprotein E and the Ischemic Brain awarded by National Institutes of Health (Principal Investigator). 1998 to 2002

same awarded by National Institutes of Health (Principal Investigator). 1996 to 2001

Integrated Training In Anesthesiology Research awarded by National Institutes of Health (Principal Investigator). 1996 to 1999

Cerebral Ischemia: Minimizing Damage During Anesthesia awarded by National Institutes of Health (Principal Investigator). 1997 to 1999

Pages

Berger, Miles, et al. “The Effect of Propofol Versus Isoflurane Anesthesia on Human Cerebrospinal Fluid Markers of Alzheimer's Disease: Results of a Randomized Trial.J Alzheimers Dis, vol. 52, no. 4, Apr. 2016, pp. 1299–310. Pubmed, doi:10.3233/JAD-151190. Full Text Open Access Copy

Eisenach, James C., et al. “Reporting of Preclinical Research in Anesthesiology: Transparency and Enforcement.Anesthesiology, vol. 124, no. 4, Apr. 2016, pp. 763–65. Pubmed, doi:10.1097/ALN.0000000000001044. Full Text

Bierens, Joost J. L. M., et al. “Physiology Of Drowning: A Review.Physiology (Bethesda), vol. 31, no. 2, Mar. 2016, pp. 147–66. Pubmed, doi:10.1152/physiol.00002.2015. Full Text

Hsieh, Justin T., et al. “Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage.Neuroendocrinology, vol. 103, no. 5, 2016, pp. 518–30. Pubmed, doi:10.1159/000440883. Full Text

Ashcraft, Kathleen A., et al. “Novel Manganese-Porphyrin Superoxide Dismutase-Mimetic Widens the Therapeutic Margin in a Preclinical Head and Neck Cancer Model.Int J Radiat Oncol Biol Phys, vol. 93, no. 4, Nov. 2015, pp. 892–900. Pubmed, doi:10.1016/j.ijrobp.2015.07.2283. Full Text

Hong, Jun, et al. “CB1 cannabinoid receptor agonist inhibits matrix metalloproteinase activity in spinal cord injury: A possible mechanism of improved recovery.Neurosci Lett, vol. 597, June 2015, pp. 19–24. Pubmed, doi:10.1016/j.neulet.2015.04.016. Full Text

Chaparro, Rafael E., et al. “Sustained functional improvement by hepatocyte growth factor-like small molecule BB3 after focal cerebral ischemia in rats and mice.J Cereb Blood Flow Metab, vol. 35, no. 6, June 2015, pp. 1044–53. Pubmed, doi:10.1038/jcbfm.2015.23. Full Text

Taninishi, Hideki, et al. “A blinded randomized assessment of laser Doppler flowmetry efficacy in standardizing outcome from intraluminal filament MCAO in the rat.J Neurosci Methods, vol. 241, Feb. 2015, pp. 111–20. Pubmed, doi:10.1016/j.jneumeth.2014.12.006. Full Text

Feix, James A., et al. “Intra-operative hydroxyethyl starch is not associated with post-craniotomy hemorrhage.Springerplus, vol. 4, 2015, p. 350. Pubmed, doi:10.1186/s40064-015-1126-0. Full Text Open Access Copy

Lombard, Frederick, et al. “Simvastatin in subarachnoid haemorrhage: beyond the short-term.Lancet Neurol, vol. 13, no. 11, Nov. 2014, p. 1073. Pubmed, doi:10.1016/S1474-4422(14)70229-7. Full Text

Pages