David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Warner, David S., et al. “Translational research in acute central nervous system injury: lessons learned and the future.Jama Neurol, vol. 71, no. 10, Oct. 2014, pp. 1311–18. Pubmed, doi:10.1001/jamaneurol.2014.1238. Full Text

Lei, Beilei, et al. “Intrastriatal injection of autologous blood or clostridial collagenase as murine models of intracerebral hemorrhage.J Vis Exp, no. 89, July 2014. Pubmed, doi:10.3791/51439. Full Text

Wang, Ruihua, et al. “ApoE mimetic ameliorates motor deficit and tissue damage in rat spinal cord injury.J Neurosci Res, vol. 92, no. 7, July 2014, pp. 884–92. Pubmed, doi:10.1002/jnr.23371. Full Text

Sheng, Huaxin, et al. “Metalloporphyrins as therapeutic catalytic oxidoreductants in central nervous system disorders.Antioxid Redox Signal, vol. 20, no. 15, May 2014, pp. 2437–64. Pubmed, doi:10.1089/ars.2013.5413. Full Text

Berger, Miles, et al. “Volatile anesthetic sedation during therapeutic hypothermia after cardiac arrest in the ICU: a journey of a thousand miles begins with a single step.Crit Care Med, vol. 42, no. 2, Feb. 2014, pp. 494–95. Pubmed, doi:10.1097/01.ccm.0000435689.93091.d0. Full Text

Berger, M., et al. “A novel application for bolus remifentanil: blunting the hemodynamic response to Mayfield skull clamp placement.Curr Med Res Opin, vol. 30, no. 2, Feb. 2014, pp. 243–50. Pubmed, doi:10.1185/03007995.2013.855190. Full Text

Warner, David S., and William L. Lanier. “Mr. Piano Man: reflections on the life of physician, scientist, and humanitarian, William L. Young, MD (1954-2013).J Neurosurg Anesthesiol, vol. 26, no. 1, Jan. 2014, pp. 1–3. Pubmed, doi:10.1097/ANA.0000000000000006. Full Text

Ishida, Kazuyoshi, et al. “Anesthetic neuroprotection: antecedents and an appraisal of preclinical and clinical data quality.Curr Pharm Des, vol. 20, no. 36, 2014, pp. 5751–65. Pubmed, doi:10.2174/1381612820666140204111701. Full Text

Lei, Beilei, et al. “Anti-inflammatory effects of progesterone in lipopolysaccharide-stimulated BV-2 microglia.Plos One, vol. 9, no. 7, 2014, p. e103969. Pubmed, doi:10.1371/journal.pone.0103969. Full Text Open Access Copy

Pages