David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Rajic, Zrinka, et al. “A new SOD mimic, Mn(III) ortho N-butoxyethylpyridylporphyrin, combines superb potency and lipophilicity with low toxicity.Free Radic Biol Med, vol. 52, no. 9, May 2012, pp. 1828–34. Pubmed, doi:10.1016/j.freeradbiomed.2012.02.006. Full Text

Sheng, Huaxin, et al. “Neuroprotective efficacy from a lipophilic redox-modulating Mn(III) N-Hexylpyridylporphyrin, MnTnHex-2-PyP: rodent models of ischemic stroke and subarachnoid hemorrhage.J Pharmacol Exp Ther, vol. 338, no. 3, Sept. 2011, pp. 906–16. Pubmed, doi:10.1124/jpet.110.176701. Full Text

Sheng, Huaxin, et al. “Pharmacologically augmented S-nitrosylated hemoglobin improves recovery from murine subarachnoid hemorrhage.Stroke, vol. 42, no. 2, Feb. 2011, pp. 471–76. Pubmed, doi:10.1161/STROKEAHA.110.600569. Full Text

Guinn, Nicole R., et al. “Adenosine-induced transient asystole for intracranial aneurysm surgery: a retrospective review.J Neurosurg Anesthesiol, vol. 23, no. 1, Jan. 2011, pp. 35–40. Pubmed, doi:10.1097/ANA.0b013e3181ef2b11. Full Text

Wang, Zhengfeng, et al. “Development of a simplified spinal cord ischemia model in mice.J Neurosci Methods, vol. 189, no. 2, June 2010, pp. 246–51. Pubmed, doi:10.1016/j.jneumeth.2010.04.003. Full Text

Pearlstein, Robert D., et al. “Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain.Int J Radiat Biol, vol. 86, no. 2, Feb. 2010, pp. 145–63. Pubmed, doi:10.3109/09553000903419965. Full Text

Borgeat, Alain, et al. “2009 in Review.” Anesthesiology, vol. 111, no. 6, Ovid Technologies (Wolters Kluwer Health), Dec. 2009, pp. 1192–96. Crossref, doi:10.1097/aln.0b013e3181c1879b. Full Text

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