David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Takata, Ken, et al. “Long-term cognitive dysfunction following experimental subarachnoid hemorrhage: new perspectives.Exp Neurol, vol. 213, no. 2, Oct. 2008, pp. 336–44. Pubmed, doi:10.1016/j.expneurol.2008.06.009. Full Text

Laskowitz, Daniel T., and David S. Warner. “The use of S100B as a biomarker in subarachnoid hemorrhage: clarity in its promise and limits.Crit Care Med, vol. 36, no. 8, Aug. 2008, pp. 2452–53. Pubmed, doi:10.1097/CCM.0b013e3181810457. Full Text

Yang, Wei, et al. “Cerebral ischemia/stroke and small ubiquitin-like modifier (SUMO) conjugation--a new target for therapeutic intervention?J Neurochem, vol. 106, no. 3, Aug. 2008, pp. 989–99. Pubmed, doi:10.1111/j.1471-4159.2008.05404.x. Full Text

Yang, Wei, et al. “Transient focal cerebral ischemia induces a dramatic activation of small ubiquitin-like modifier conjugation.J Cereb Blood Flow Metab, vol. 28, no. 5, May 2008, pp. 892–96. Pubmed, doi:10.1038/sj.jcbfm.9600601. Full Text

Yang, Wei, et al. “Transient global cerebral ischemia induces a massive increase in protein sumoylation.J Cereb Blood Flow Metab, vol. 28, no. 2, Feb. 2008, pp. 269–79. Pubmed, doi:10.1038/sj.jcbfm.9600523. Full Text

James, Michael Lucas, et al. “Preclinical models of intracerebral hemorrhage: a translational perspective.Neurocrit Care, vol. 9, no. 1, 2008, pp. 139–52. Pubmed, doi:10.1007/s12028-007-9030-2. Full Text

Laskowitz, Daniel T., and David S. Warner. “Statins in acute brain injury: getting the cart before the horse.Neurocrit Care, vol. 8, no. 1, 2008, pp. 3–5. Pubmed, doi:10.1007/s12028-007-9040-0. Full Text

McDonagh, David L., and David S. Warner. “Hypertonic saline for craniotomy?Anesthesiology, vol. 107, no. 5, Nov. 2007, pp. 689–91. Pubmed, doi:10.1097/01.anes.0000286923.77106.63. Full Text

Warner, David S. “Debra A. Schwinn, M.D., Recipient of the 2007 Excellence in Research Award.” Anesthesiology, vol. 107, no. 3, Ovid Technologies (Wolters Kluwer Health), Sept. 2007, pp. 380–82. Crossref, doi:10.1097/01.anes.0000279490.10778.6d. Full Text

Fukuda, S., and D. S. Warner. “Cerebral protection.Br J Anaesth, vol. 99, no. 1, July 2007, pp. 10–17. Pubmed, doi:10.1093/bja/aem140. Full Text

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