David Samuel Warner

David Samuel Warner

Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine

External Address: 
303 Research Dr., 134 Sands Buildign, Durham, NC 27710
Internal Office Address: 
Box 3094 Med Ctr, Durham, NC 27710
Phone: 
919.684.6633

Overview

Humans may sustain a variety of forms of acute central nervous system injury including ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection Laboratories is dedicated to examining the pathophysiology of acute brain and spinal cord injury with particular reference to disease states managed in the perioperative or neurointensive care environments. Rodent recovery models of cerebral ischemia, traumatic brain injury, cardiopulmonary bypass, subarachnoid hemorrhage, spinal cord ischemia, and perinatal hypoxia have been established with requisite control of relevant physiologic variables. Experimental protocols examine the response of brain to these insults and seek to define appropriate therapeutic interventions. Our work examines the role reactive oxygen species in CNS injury with emphasis on how pharmacologic or genetic variants modulate these processes. Effects of altered synthesis of superoxide dismutase and apolipoprotein E are investigated in transgenic/knock out mice. Outcome studies allow definition of efficacy of pharmacologic agents including superoxide dismutase mimetics, PARP-1 inhibitors, hepatocyte growth factor mimetics, recombinant apolipoprotein E and its peptide fragments, SNO-hemoglobin, and anesthetics on histologic and behavioral recovery from ischemic/traumatic insults. Recent focus has been on SUMOylation responses of neural tissue to ischemic stress. Neurochemical, immunohistochemical, molecular biologic, genomic, and proteomic techniques are used to define the mechanistic basis of observations made in outcome studies. Primary neuronal/glial cultures, organotypic hippocampal slices and immortalized transfected human cell lines are used investigate mechanistic interactions between pharmacologic agents and metabolic stresses.

Education & Training

  • M.D., University of Wisconsin - Madison 1980

Wang, Haichen, et al. “Simvastatin and atorvastatin improve behavioral outcome, reduce hippocampal degeneration, and improve cerebral blood flow after experimental traumatic brain injury.Exp Neurol, vol. 206, no. 1, July 2007, pp. 59–69. Pubmed, doi:10.1016/j.expneurol.2007.03.031. Full Text

Rice, Henry E., et al. “Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice.Ajr Am J Roentgenol, vol. 188, no. 4, Apr. 2007, pp. 1101–08. Pubmed, doi:10.2214/AJR.06.0663. Full Text

Wang, H., et al. “An apolipoprotein E-based therapeutic improves outcome and reduces Alzheimer's disease pathology following closed head injury: evidence of pharmacogenomic interaction.Neuroscience, vol. 144, no. 4, Feb. 2007, pp. 1324–33. Pubmed, doi:10.1016/j.neuroscience.2006.11.017. Full Text

Bierens, J., et al. Drowning. Jan. 2007, pp. 1088–102. Scopus, doi:10.1017/CBO9780511544828.063. Full Text

Sakai, Hiroaki, et al. “Isoflurane provides long-term protection against focal cerebral ischemia in the rat.Anesthesiology, vol. 106, no. 1, Jan. 2007, pp. 92–99. Pubmed, doi:10.1097/00000542-200701000-00017. Full Text

Warner, D. S., and J. T. A. Knape. Brain resuscitation in the drowning victim task force on brain resuscitation. Dec. 2006, pp. 435–78. Scopus, doi:10.1007/3-540-29656-5_8. Full Text

Wise-Faberowski, Lisa, et al. “NMDA-induced apoptosis in mixed neuronal/glial cortical cell cultures: the effects of isoflurane and dizocilpine.J Neurosurg Anesthesiol, vol. 18, no. 4, Oct. 2006, pp. 240–46. Pubmed, doi:10.1097/00008506-200610000-00004. Full Text

Mesis, Rachel G., et al. “Dissociation between vasospasm and functional improvement in a murine model of subarachnoid hemorrhage.Neurosurg Focus, vol. 21, no. 3, Sept. 2006, p. E4. Pubmed, doi:10.3171/foc.2006.21.3.4. Full Text

Wang, James K. T., et al. “Cardiac glycosides provide neuroprotection against ischemic stroke: discovery by a brain slice-based compound screening platform.Proc Natl Acad Sci U S A, vol. 103, no. 27, July 2006, pp. 10461–66. Pubmed, doi:10.1073/pnas.0600930103. Full Text

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