Vadim Y Arshavsky
Helena Rubinstein Foundation Distinguished Professor of Ophthalmology
Research conducted in our laboratory is dedicated to understanding how vision is performed on the molecular level. Our most mature direction addresses the function of rod and cone photoreceptors, which are sensory neurons responsible for the detection and primary processing of information entering the eye in the form of photons. Photoreceptors respond to capturing photons by generating electrical signals transmitted to the secondary neurons in the retina and, ultimately, to the brain. Our work is dedicated to uncovering the molecular mechanisms underlying three essential photoreceptor functions: their uniquely high light-sensitivity, their ability to rapidly recover from light excitation, and their capacity to modulate light-responses upon broad variations in the intensity of ambient illumination.
Our second direction is to elucidate the cellular processes responsible for building the light-sensitive organelle of photoreceptor cells, called the outer segment, and for populating this organelle with proteins conducting visual signaling. Of particular interest is the mechanism by which outer segments form their “disc” membrane stacks providing vast membrane surfaces for effective photon capture.
Finally, we are seeking connections between understanding the basic function of rods and cones and practical, translational approaches to ameliorate the retinal degeneration caused by mutations in critical photoreceptor-specific proteins. Most importantly, we explore the link between the balance of protein synthesis and degradation in photoreceptor cells (the “proteostasis”) and the status of their health.
Molecular mechanisms of photoreceptor outer segment morphogenesis awarded by National Institutes of Health (Principal Investigator). 2005 to 2023
In Vivo Modeling of Mitochondrial Complex I Deficiency in Retinal Ganglion Cells awarded by National Institutes of Health (Mentor). 2018 to 2023
Pathological and functional consequences of dimerization-deficient rhodopsin mutations awarded by National Institutes of Health (Principal Investigator). 2019 to 2022
Center Core Grant for Vision Research awarded by National Institutes of Health (Principal Investigator). 2001 to 2021
Repulsive mechanisms for spatial segregation of developing neural circuits awarded by National Institutes of Health (Co-Sponsor). 2017 to 2020
The Role of Basal Bodies in Wnt Signaling awarded by National Institutes of Health (Collaborator). 2007 to 2020
Pharmacological Sciences Training Program awarded by National Institutes of Health (Participating Faculty Member). 1975 to 2020
Organization and Function of Cellular Structure awarded by National Institutes of Health (Mentor). 1975 to 2020
Rhodopsin dimerization: mechanistic basis and functional consequences awarded by (Principal Investigator). 2017 to 2020
Coherent light scattering for early detection of Alzheimer's disease awarded by National Institutes of Health (Co Investigator). 2017 to 2020
Herrmann, R., and V. Y. Arshavsky. “The role of dopamine in fine-tuning cone- and rod-driven vision.” G Protein Signaling Mechanisms in the Retina, 2014, pp. 121–41. Scopus, doi:10.1007/978-1-4939-1218-6_8. Full Text
Calvert, P. D., and V. Y. Arshavsky. “Light-driven translocation of signaling proteins in vertebrate photoreceptors.” Encyclopedia of the Eye, 2010, pp. 577–80. Scopus, doi:10.1016/B978-0-12-374203-2.00176-7. Full Text
Perfilov, Maxim M., et al. “Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization.” Cell Mol Life Sci, Jan. 2020. Pubmed, doi:10.1007/s00018-019-03426-5. Full Text
Spencer, William J., et al. “Photoreceptor disc membranes are formed through an Arp2/3-dependent lamellipodium-like mechanism.” Proc Natl Acad Sci U S A, Dec. 2019. Pubmed, doi:10.1073/pnas.1913518117. Full Text
Lobanova, Ekaterina S., et al. “Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction.” J Neurosci, vol. 39, no. 49, Dec. 2019, pp. 9689–701. Pubmed, doi:10.1523/JNEUROSCI.1142-19.2019. Full Text
Gospe, Sidney M., et al. “Photoreceptors in a mouse model of Leigh syndrome are capable of normal light-evoked signaling.” J Biol Chem, vol. 294, no. 33, Aug. 2019, pp. 12432–43. Pubmed, doi:10.1074/jbc.RA119.007945. Full Text Open Access Copy
Spencer, William J., et al. “PRCD is essential for high-fidelity photoreceptor disc formation.” Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 26, June 2019, pp. 13087–96. Epmc, doi:10.1073/pnas.1906421116. Full Text
O’Koren, Emily G., et al. “Microglial Function Is Distinct in Different Anatomical Locations during Retinal Homeostasis and Degeneration.” Immunity, vol. 50, no. 3, Mar. 2019, pp. 723-737.e7. Pubmed, doi:10.1016/j.immuni.2019.02.007. Full Text
Spencer, William J., and Vadim Y. Arshavsky. “PRCD Is a Small Disc-Specific Rhodopsin-Binding Protein of Unknown Function.” Adv Exp Med Biol, vol. 1185, 2019, pp. 531–35. Pubmed, doi:10.1007/978-3-030-27378-1_87. Full Text
Dexter, Paige M., et al. “Transducin β-Subunit Can Interact with Multiple G-Protein γ-Subunits to Enable Light Detection by Rod Photoreceptors.” Eneuro, vol. 5, no. 3, May 2018. Epmc, doi:10.1523/ENEURO.0144-18.2018. Full Text Open Access Copy
Lobanova, Ekaterina S., et al. “Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration.” Nat Commun, vol. 9, no. 1, Apr. 2018, p. 1738. Pubmed, doi:10.1038/s41467-018-04117-8. Full Text Open Access Copy
Sharif, Ali S., et al. “C8ORF37 Is Required for Photoreceptor Outer Segment Disc Morphogenesis by Maintaining Outer Segment Membrane Protein Homeostasis.” J Neurosci, vol. 38, no. 13, Mar. 2018, pp. 3160–76. Pubmed, doi:10.1523/JNEUROSCI.2964-17.2018. Full Text
Ray, Thomas, et al. “CRB1 expresses multiple isoforms in multiple retinal cell types - reevaluating CRB1 retinopathies.” Investigative Ophthalmology & Visual Science, vol. 60, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
Lobanova, Katya, et al. “Reduced docosahexaenoic acid content impairs photoreceptor health without affecting visual signal transduction.” Investigative Ophthalmology & Visual Science, vol. 60, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
Arshavsky, Vadim Y. “How to build a photoreceptor disc?” Investigative Ophthalmology & Visual Science, vol. 60, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
Spencer, William, et al. “PRCD supports the organized structure of the photoreceptor outer segment.” Investigative Ophthalmology & Visual Science, vol. 60, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
Pearring, Jillian Nydam, et al. “Ciliary Delivery of the CNG-gated Channel in Rod Photoreceptors.” Investigative Ophthalmology & Visual Science, vol. 60, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2019.
Skiba, Nikolai P., et al. “Identification of protein components of the rod outer segment plasma membrane by label-free protein correlation profiling.” Investigative Ophthalmology & Visual Science, vol. 59, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2018.
Maddala, Rupalatha, et al. “Deficiency of S100A4, a Ca2+-binding protein, Induces Expression of Neuronal S100A5 and the Retinal Specific Transcriptome in mouse lens.” Investigative Ophthalmology & Visual Science, vol. 59, no. 9, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2018.
Lobanova, Ekaterina, et al. “An increase in proteolytic capacity delays photoreceptor loss in retinal degeneration.” Investigative Ophthalmology & Visual Science, vol. 58, no. 8, ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2017.
Pearring, JN, San Agustin, JT, Lobanova, ES, Gabriel, CJ, Monis, WJ, Lieu, EC, Stuck, MW, Arshavsky, VY, and Pazour, GJ. "Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration." 2017.
Baker, Sheila A., et al. “Investigating Synaptophysin Targeting to the Photoreceptor Synapse.” Faseb Journal, vol. 26, 2012.
Tate, R. J., et al. “Erratum: The identification of the inhibitory γ-subunits of the type 6 retinal cyclic guanosine monophosphate phosphodiesterase in non-retinal tissues: Differential processing of mRNA transcripts (Genomics (2002) 79 (582-586)).” Genomics, vol. 83, no. 2, 1 Jan. 2004. Scopus, doi:10.1016/j.ygeno.2003.08.001. Full Text